NerdRx Podcast

NerdRx Podcast

Hello everyone, NerdRx podcast brings you a new scientific technique every week. I will invite scientists from various backgrounds and discuss their favorite lab techniques. Stay tuned every Tuesday for a new episode. If you have any topic suggestions or if you would like to join me for an episode please email me at barkha@nerdrxpodcast.com

Episodes

August 1, 2023 32 mins

Hello listeners,
Today is an exciting one, Microscopy. Out guest Dr. Saurabh Mehta will discuss the differences between light, confocal and super resolution microscopy. And wait till you hear the fun fact about microscopy. Thank you for joining us, and I hope you keep listening.

Reading suggestions:
Nikon MicroscopyU. The source for microscopy education
https://www.microscopyu.com

 Dr. Eric Betzig – UC Berkele...

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Hello listeners,
This week on NerdRx podcast, we have a novel cell culture technique called bacteria spheroid co-cultures. Dr. Deb will discuss how these bacteria thrive within cancerous tumors and can be used as therapies in the future. Thank you for joining us, and I hope you keep listening.

Reading suggestions:
A rapid screening platform to coculture bacteria within tumor spheroids
https://www.nature.com/a...

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Hello listeners, 

This week we will discuss a behavior technique different from what we usually discuss. Our guest Subhajit Sarkar, a PhD student at IIT Kharagpur in India, will talk about the nitty-gritty of how to perform memory assays in a fruit fly!

Thank you for joining us, and I hope you keep listening. 


Reading suggestions:

Plasticity of local GABAergic interneurons drives olfactory habituatio...

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July 11, 2023 28 mins

Hello listeners, 
Today we will talk about BRET, which is short for Bioluminescence Resonance Energy Transfer, with a fellow content creator and podcaster, David Lakhter. Hear it from David about his journey and how he got into creating content. Thank you for joining us, and I hope you keep listening. 

Reading suggestions:

Application of BRET to monitor ligand binding to GPCRs

https://www.ncbi.nlm.nih.gov/pmc/articles/PM...

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Hello listeners, 
I am so excited that this week my former mentor and the person who made me fall in love with Chemistry is with us tonight. This podcast began with high-throughput screening and Dr. Malaika Argade will take a step further and discuss what happens next. Thank you for joining us, and I hope you keep listening. 

Email me your suggestions at barkha@nerdrxpodcast.com

Website: https://www.nerdrxpodcast.com/

RSS Feed: ht...

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Hello listeners, 

This week we are going to discuss how scientists use sound waves to dispense liquids at a nano scale. Joining us for this episode is Dr. Julia Hottinger. Thank you for joining us, and I hope you keep listening. 

 Reading suggestion:
A review on acoustic droplet ejection technology and system
https://pubs.rsc.org/en/content/articlelanding/2021/sm/d0sm02193h

Acoustic Droplet Ejection Applications for ...

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Hello listeners, 

I have been waiting for this one for a long time, CRISPR/Cas9. In this episode, our guest Dr. Carla Belmonte gives us the details of the beauty of CRISPR/Cas9 and how it is evolving as a therapy. Thank you for joining us, and I hope you keep listening. 

Reading suggestion:
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
https://www.science.org/doi/full/10.1126/science.1225829

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Hello listeners, 

This week Dr. Thomas Bonacci will talk about the method he uses to study every stage of cell cycle. Yes, going to the basics with Cell-free systems.  Thank you for joining us, and I hope you keep listening. 

Reading suggestions:

What makes the cell cycle tick? a celebration of the awesome power of biochemistry and the frog egg

https://pubmed.ncbi.nlm.nih.gov/33320710/

 

Email...

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Hello listeners,
We all have hear about cell cultures but do you that there are 3D-cell cultures too? This week we have Dr. Nishant Kulkarni, who will talk all about his experience with 3D cell cultures. Thank you for joining us, and I hope you keep listening.

Reading suggestions:
Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning
https://www.ncbi.nlm.nih.gov/pmc/articles/...

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Hello listeners, 

This week Dr. Crystal Starbird, Assistant Professor at UNC Chapel Hill will talk about X-Ray Crystallography, a rival to CryoEM. Thank you for joining us, and I hope you keep listening. 

Reading Suggestion:
RCSB Protein Data Bank
https://www.rcsb.org 

Email me your suggestions at barkha@nerdrxpodcast.com

Website: https://www.nerdrxpodcast.com/

RSS Feed: https://feeds.buzzsprout.com/2051636.rss

Please follow Nerd...

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April 18, 2023 41 mins

Hello listeners,
Well if you thought only clothes or shoes can be designers, you are wrong! This week Sophie Bendrath will tell us about a fascinating technique of Designer Receptors Exclusively Activated by Designer Drugs aka DREADDs. Thank you for joining us, and I hope you keep listening.

Reading suggestion:
DREADDs for Neuroscientists
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759656/ 

Email me your sug...

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Hello listeners, 

Today we will talk about one of my favorite techniques, Chromatography. Join me with Dr. Abha Jain, a postdoc at UNC Chapel Hill, for this episode, where we discuss three different types of chromatography, ion exchange, size exclusion, and affinity chromatography. Thank you for joining us, and I hope you keep listening. 

Reading suggestion:

Separation techniques: Chromatography
https://www.ncbi.nlm.nih.gov/pmc/ar...

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April 4, 2023 38 mins

Hello listeners, 

This week we have a UNC Ph.D. candidate Alexis Stutzman. Alesis will dive into a genomic technique called Hi-C that is used to measure DNA-DNA contacts. Thank you for joining us, and I hope you keep listening. 

Reading suggestion:

Advancements in Mapping 3D Genome Architecture
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7391055/

Chromatin Architecture Emerges during Zygotic Genome Activation Independent...

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Hello listeners, 

For our March special, we dive into something new. Dr. Sharena Rice will walk us through Neurotechnology and how it can be life-changing and can be used in treating so many issues, we are currently facing. Thank you for joining us, and I hope you keep listening. 

Email me your suggestions at barkha@nerdrxpodcast.com

Website: https://www.nerdrxpodcast.com/

RSS Feed: https://feeds.buzzsprout.com/2051636.rss

Please follow...

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Hello listeners, 

This week we have Christa Frodella who will be discussing the technical side of RNA sequencing. RNAseq is a technique used to quantify RNA in a biological sample and has the ability to look at gene fusion, post translation modifications and more. Thank you for joining us, and I hope you keep listening.

Email me your suggestions at barkha@nerdrxpodcast.com

Website: https://www.nerdrxpodcast.com/

RS...

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Hello listeners, 

This week we discuss immunocytochemistry (ICC) with Dr. Hallie Blevins who I know from my graduate school at VCU. ICC is a great way to identify and quantify the distribution of your target proteins and is routinely used in many labs. Thank you for joining us, and I hope you keep listening. 

 Reading suggestions:
Immunocytochemistry and quantification of protein colocalization in cultured neurons
https://www...

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Hello listeners, 

This week we have Dr. Emily Witt, who discusses a behavioral technique called Self-administration and how do we use this technique to understand the abuse liability of a molecule. Thank you for joining us, and I hope you keep listening. 

Reading suggestions:
Behavioural assessment of drug reinforcement and addictive features in rodents: an overview
https://pubmed.ncbi.nlm.nih.gov/16759333/

Support t...

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Hello listeners, 

You do not want to miss it. We hear so many stories about cannabinoids these days but do you know how the cannabinoid receptor was discovered and the story behind it? Join me for this week’s episode where we have the scientist who actually discovered the cannabinoid receptor, Dr. Allyn Howlett. Thank you for joining us, and I hope you keep listening.

Support this podcast: https://www.buymeacoffee.com/nerd...

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Hello listeners, 

We all would love if we lived our lives without any fear but did you know that fear is our survival mechanism? This week on NerdRx podcast we have Dr. Ian Jacobs who will discuss the ins and out of how do we study fear in a lab setting. Thank you for joining us, and I hope you keep listening. 

 Reading suggestions:
Context, Ambiguity, and Unlearning: Sources of Relapse after Behavioral Extinction https://www.app...

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Hello listeners, 

This week allow me to take you back to the basics of Thermodynamics. We have Dr. Shashank Chavali for our discussion on ITC and find out at the end and find out which disease utilized ITC to help develop treatment. Thank you for joining us, and I hope you keep listening. 

Reading suggestions:

Cyclic peptides with a distinct arginine-fork motif recognize the HIV trans-activation response RNA in vi...

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