3 Minute 3Rs

January 2023

The papers behind the pod:

1. Cassotta M et al. (2022). A worldwide survey on the use of animal-derived materials and reagents in scientific experimentation. Engineering in Life Sciences 22(9):561-604. doi: 10.1002/elsc.202100167  
2. Nuber M et al. (2022). Development of a 3D simulator for training the mouse in utero electroporation. PLOS One ...

December 2022

The papers behind the pod:

1. Menéndez ABC et al. (2022). Creating a kidney organoid-vasculature interaction model using a novel organ-on-chip system. Scientific Reports 12:20699. doi: 10.1038/s41598-022-24945-5
2. Karp N et al. (2022). A qualitative study of the barriers to using blinding in in vivo experiments and suggestions for improvement. PLOS Biolo...

November 2022

The papers behind the pod:

1. Aulehner K et al. (2022). Grimace scale, burrowing, and nest building for the assessment of post-surgical pain in mice and rats—A systematic review. Frontiers in Veterinary Science 9:930005. doi: 10.3389/fvets.2022.930005
2. Hair K et al. (2022). ‘Living’ evidence frameworks for in vivo animal research: towards translational evidence-b...

October 2022

The papers behind the pod:

1. Piper, SK et al. (2022). Statistical review of animal trials—A guideline. Biometrical Journal https://doi.org/10.1002/bimj.202200061
2. Gargaté MJ et al. (2022). Parallel Propagation of Toxoplasma gondii In Vivo, In Vitro and in Alternate Model: Towards Less Dependence on the Mice Model. Pathogens https://doi.org/10.3390/pathogens11091038
3. Mieske P et al. et al...

September 2022

The papers behind the pod:

1. Pinho RH et al. (2022). Validation of the rabbit pain behaviour scale (RPBS) to assess acute postoperative pain in rabbits (Oryctolagus cuniculus). PLoS ONE 17(5): e0268973. https://doi.org/10.1371/journal.pone.0268973
2. Stull C, Heagerty A and Coleman K (2022). Video Conference Technology as a Tool for Pair Introduction in Rhesus Macaques. Animals&nbs...

August 2022

The papers behind the pod:

1. Arjmand S et al. (2022). Tips and traps for behavioural animal experimentation. Acta Neuropsychiatrica, in press. https://doi.org/10.1017/neu.2022.4
2. Wrede P et al. (2022). Real-time 3D optoacoustic tracking of cell-sized magnetic microrobots circulating in the mouse brain vasculature. Science Advances 8(19). https://doi.org/10.1126/sc...

July 2022

The papers behind the pod:

1. Ferreira DA et al. (2021). Alternative to Soft Lithography for the Fabrication of Organ-on-a-Chip Elastomeric-Based Devices and Microactuators. Advanced Science 8:2003273. 
2. https://doi.org/10.1002/advs.202003273
3. Eigel D et al. (2019). Cryogel scaffolds for regionally constrained delivery of lysophosphatidylcholine to central nervous system slice cultures: A mod...

June 2022

The papers behind the pod:

1. Williams WO and Baneux P (2022). Humane Intervention Points: Refining endpoint terminology to incorporate non-euthanasia intervention options to improve animal welfare and preserve experimental outcomes. Laboratory Animals, in press. https://doi.org/10.1177/00236772221090801
2. Osorio D et al. (2022). scTenifoldKnk: An efficient virtual knockout tool for gene fun...

May 2022

The papers behind the pod:

1. Wang, Q et al. (2022). Assessment of a 3D neural spheroid model to detect pharmaceutical-induced neurotoxicity. ALTEX 39. https://doi.org/10.14573/altex.2112221
2.  Reynolds P.S. (2022) Between two stools: preclinical research, reproducibility, and statistical design of experiments. BMC Res. Notes 15, 73.  https://doi.org/10.1186/s13104-022-05965...

April 2022

The papers behind the pod:

1. Nigri M et al. (2022). Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice. Frontiers in Behavioural Neuroscience 16: e835444. https://doi.org/10.3389/fnbeh.2022.835444
2.  Vámos A et al. (2022) Mitophagy Mediates the Beige to White Transition of Human Primary Subcutaneous Adipocytes Ex Vivo.&nb...

The papers behind the pod:

1.      Baran SW et al. (2022). Perspectives on the Evaluation and Adoption of Complex In Vitro Models in Drug Development: Workshop with the FDA and the Pharmaceutical Industry (IQ MPS Affiliate). ALTEX, in press. https://doi.org/10.14573/altex.2112203

2.      Borba JVB et al. (2022) STopTox: An in Silico Alternative to Animal Testing for Acu...

February

https://doi.org/10.1186/s12915-021-01184-0

https://doi.org/10.1177/00236772211065920

https://doi.org/10.1038/s41598-021-04502-2

It’s the 3rd Thursday of February, and you’re listening to 3 Minute 3Rs. This month, we’re bringing you 3 refinement papers. Let’s start with rodent housing.

Research rodents are conventionally housed in shoebox sized cages that limit their ability to perform natural behaviors such as nesting ...

The papers behind the pod:

1. https://doi.org/10.7554/eLife.71601 & https://doi.org/10.7554/eLife.67995

2. https://doi.org/10.3389/fnins.2021.805679

3. https://doi.org/10.1038/s41598-021-98356-3

It’s the 3rd Thursday of January – happy new year! You’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce and refine the use of animals in research. Of course, we focus on those three Rs, but many have sugges...

Sedation is necessary for zebrafish during procedures such as imaging, biopsy, and surgery to ensure animal welfare and high-quality science. But the effects of sedation can last beyond the administration period and should also be carefully considered. A paper by Gressler et al. explores the use of eugenol and propofol during a 3-hour sedation and their subsequent effects after a 1 hour washout period. Both drugs had effects on beh...

The papers behind the pod:

1. A non-human primate in vitro functional assay for the early evaluation of TB vaccine candidates. npj Vaccines, January 2021. https://doi.org/10.1038/s41541-020-00263-7

2. Inducible dominant negative ErbB2 rat spermatogonial line for generation of transgenic rat model and dissecting ERBB2 tyrosine kinase mediated pathways. Experimental Oncology, June 2019. https://doi.org/10.32471/exp-oncolo...

The papers behind the pod:

1. Professional Quality of Life in Research Involving Laboratory Animals. Animals 11(9), 2639 (2021) https://doi.org/10.3390/ani11092639

2. Improving culture of care through maximising learning from observations and events: Addressing what is at fault. Laboratory Animals (2021). https://doi.org/10.1177%2F00236772211037177

3. Epidemiology and reporting characteristics of preclinical systematic reviews. PLOS B...

Sept 2021 

You’re listening to the September episode of 3 Minute 3Rs. 

The papers behind the pod: 

1. Performance of preclinical models in predicting drug-induced liver injury in humans: a systematic review. Scientific Reports 11, 6403 (2021) https://doi.org/10.1038/s41598-021-85708-2 
2. Ratlas-LH: An MRI template of the Lister hooded rat brain with stereotaxic coordinates for neurosurgical impla...

You’re listening to the August episode of 3 Minute 3Rs!

The papers behind the pod: 

1. Refinement of the spinal cord injury rat model and validation of its applicability as a model for memory loss and chronic pain. Heliyon 7, E07500 (2021) https://doi.org/10.1016/j.heliyon.2021.e07500 
2. Strategies to encourage the adoption of social housing during cardiovascular telemetry recordings in non-rodents.&nb...

It’s the 3rd Thursday of July and you’re listening to 3 Minute 3Rs, your monthly recap of efforts to replace, reduce, and refine the use of animals in research. 

The papers behind the pod: 

• SARS-CoV-2 infects an upper airway model derived from induced pluripotent stem cells. Stem Cells https://stemcellsjournals.onlinelibrary.wiley.com/doi/full/10.1002/stem.3422
• A break from the pups: The effects of lof...