Protein Colocalization: 2 Essential Methods to Prove Protein Overlap

#58 — One of the most common goals in biology is to prove that two proteins colocalize. Usually, colocalization happens as part of a crucial disease pathway or poorly understood yet fundamental physiological process.

In this episode, we discuss two methods to quantify colocalization: Pearson’s correlation coefficient and Mander’s colocalization coefficient. Listen and learn their use cases and limitations and how to choose appropriate regions of interest in each case.

Read the full article for helpful figures, [1] and because observer subjectivity and color blindness can skew results, check out this article on accessibility in science. [2]

Resources:
1. Protein Colocalization: 2 Essential Methods to Prove Protein Overlap. Available at: https://bitesizebio.com/38330/protein-colocalization-you-know-they-overlap-but-can-you-prove-it/
2. Accessibility in Science: How Can We Make Science More Accessible? Available at: https://bitesizebio.com/65863/accessibility-in-science/